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Patent US10724048
Trait Biosciences, Inc.

Systems And Methods For Enhancing Trichome Formation And Density In Cannabis

The present invention includes systems, methods and compositions for increasing trichome formation and/or density in cannabinoid producing plants, such as Cannabis and hemp. Additional aspects of the invention further include systems, methods and compositions for increasing cannabinoid and terpene biosynthetic and storage capacity in Cannabis.

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1 Independent Claims

  • Claim CLM-00001. 1. A method of increasing trichome formation in a plant comprising the steps: stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence according to SEQ ID NO. 7, wherein expression of the heterologous polynucleotide sequence independently upregulates trichome formation and metabolic flux through the cannabinoid biosynthetic pathway in said cannabinoid producing plant; and wherein said cannabinoid producing plant is Cannabis.
  • Claim CLM-00011. 11. A method of increasing cannabinoid formation in a plant comprising the steps: stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence according to SEQ ID NO. 7, wherein expression of the heterologous polynucleotide sequence independently upregulates metabolic flux through the cannabinoid biosynthetic pathway increasing formation of CBDA and/or THCA in said cannabinoid producing plant and independently upregulates trichome formation in said cannabinoid producing plant; and wherein said cannabinoid producing plant is Cannabis.
  • Claim CLM-00020. 20. A method of increasing trichome formation in a plant comprising the steps: stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence encoding transcription factor MYB1 from Artemisia annua, wherein expression of the heterologous MYB1 independently upregulates trichome formation and metabolic flux through the cannabinoid biosynthetic pathway in said cannabinoid producing plant; and wherein said cannabinoid producing plant is Cannabis.


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