Application US20200107511

SELECTION OF NEW VARIETIES OF CANNABIS PLANTS EXPRESSING CANNABINOIDS BY CELL CULTURE
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US20200107511

1. A method for generating new varieties of Cannabis plants with modified growth and cannabinoid phytochemical profiles, comprising: (5) (3)

subjecting plant parts of one or more Cannabis plants to pectinase digestion to release plant cells;

centrifuging the released plant cells to obtain pelleted cells; and

plating the pelleted cells on a first type of culture media, which is a Murashige and Skoog or Gamborg B5 callus culture media.

2. The method of claim 1, comprising, prior to the subjecting: (1) (2)

cleansing the plant parts plants, said plant parts including mature leaves and floral parts; and

cutting said plant parts into pieces of approximately 1 cm in length after said cleansing.

3. The method of claim 2, wherein said cleansing comprises: (0) (2)

4. The method of claim 1, wherein said subjecting the plant parts to pectinase digestion comprises: (0) (2)

5. The method of claim 1, comprising, after said subjecting and prior to said centrifuging: (0) (2)

6. The method of claim 1, wherein said centrifuging is performed at 500-1000 G. (0)

7. The method of claim 1, comprising: (2) (2)

incubating the pelleted cells using different sets of growth conditions to establish growth; and

transferring calli from the first type of culture media to a second type of culture media for additional growth.

8. The method of claim 7, wherein the second type of culture media is root and shoot media. (0)

9. The method of claim 7, wherein said incubating the pelleted cells using different sets of growth conditions comprises: (2) (2)

incubating a first portion of the pelleted cells under a first set of growth conditions; and

incubating a second portion of the pelleted cells under a second set of growth conditions that differ from the first set of growth conditions and provide a greater degree of stress than the first set of growth conditions.

10. The method of claim 9, wherein the second set of growth conditions include at least one of water stress, saline conditions, and temperature conditions. (0)

11. The method of claim 9, comprising: (1) (2)

obtaining plantlets from the second type of culture media grown from the first portion and second portion of pelleted cells; and

planting the plantlets in biodegradable pots at least partially composed of peat moss and wood pulp.

12. The method of claim 11, comprising: (4) (2)

analyzing a growth pattern and cannabinoid profile of the plantlets as the plantlets grow into plants; and

selecting ones of the plants for further propagation based on a growth characteristic and a Cannabinoid characteristic.

13. The method of claim 12, comprising further basing said selecting on a water response of the plants, a temperature resistance of the plants, a salinity tolerance of the plants, and a flowering time of the plants. (0)

14. The method of claim 12, wherein the growth characteristic comprises a height of a plant being below a predefined threshold. (1) (0)

16. The method of claim 12, comprising: (0) (1)

17. The method of claim 12, comprising: (0) (2)

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Application US20200107511

SELECTION OF NEW VARIETIES OF CANNABIS PLANTS EXPRESSING CANNABINOIDS BY CELL CULTURE

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