Application US20190041391

USE OF CIRCULATING MICROPARTICLES TO STRATIFY RISK OF SPONTANEOUS PRETERM BIRTH
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US20190041391

1. A method for assessing risk of spontaneous preterm birth for a pregnant subject, the method comprising: (6) (3)

a. preparing a microparticle-enriched fraction from a blood sample from the pregnant subject;

b. determining a quantitative measure of a panel of microparticle-associated proteins in the fraction, wherein the panel comprises at least three proteins selected from the proteins of Table 1 or Table 2; and

c. assessing the risk of spontaneous preterm birth based on the measure.

2. The method of claim 1 wherein the panel comprises at least three proteins selected from the proteins of Table 4 or Table 5. (0)

20. The method of claim 1, wherein the pregnant subject is a primigravida female. (0)

21. The method of claim 1, wherein the sample is taken from the pregnant subject during the first or second trimester. (0)

26. The method of claim 1, wherein the blood sample is a serum or plasma sample. (0)

27. The method of claim 1, wherein the microparticle-enriched fraction is prepared using size-exclusion chromatography. (4) (0)

32. The method of claim 1, wherein determining a quantitative measure comprises mass spectrometry. (1) (0)

3.-19. (canceled) (0)

22.-25. (canceled) (0)

35.-43. (canceled) (0)

44. A method comprising administering to a pregnant subject characterized as having a panel of microparticle-associated proteins indicative of an increased risk of spontaneous preterm birth, an effective amount of a treatment designed to reduce the risk of spontaneous preterm birth, wherein the panel comprises at least three proteins selected from the proteins of Table 1 or Table 2. (2) (0)

47. A method comprising: (1) (2)

49.-78. (canceled) (0)

79. A method comprising: (0) (3)

a) preparing a microparticle-enriched fraction from plasma or serum of a pregnant subject at from 8 to 14 weeks of gestation;

b) using selected reaction monitoring mass spectrometry, determining a quantitative measure of one or more proteins in the fraction, wherein the proteins are selected from the proteins of Tables 1, 2, 4, 5, 7, 8, or 9; and

c) executing a classification rule of a classification system which rule, based on values including the quantitative measures, classifies the subject as being at risk of spontaneous preterm birth, wherein the classification system, in a receiver operating characteristic (ROC) curve, has an area under the curve (AUC) of at least 0.6.

80.-85. (canceled) (0)

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Application US20190041391

USE OF CIRCULATING MICROPARTICLES TO STRATIFY RISK OF SPONTANEOUS PRETERM BIRTH

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