Abstract: |
The invention relates to novel genetically modified plants and methods or materials, such as polynucleotides, expression cassettes, or vectors for producing the same. Moreover, the invention relates to altering the content of cannabinoids in plants and to medical compositions derived from such plants. In particular embodiments, the present invention relates to cannabis plants having modified expression of tetrahydrocannabinolic acid (THCA) synthase and methods of modifying the amount of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) in cannabis by modifying expression of THCA synthase. |
Inventor: |
Boudko, Ekaterina Alexandra (Smiths Falls, CA); Shipley, Thomas IV (Smiths Falls, CA); Johnson, Douglas (Ottawa, CA) |
Applicant: |
Tweed, Inc. (Smiths Falls, CA) |
Face Assignee: |
Tweed, Inc. (Smiths Falls, CA) |
Filed: |
2016-05-27 |
Issued: |
2021-03-02 |
Claims: |
13 |
|
US10934554
|
1. A method for altering a content of one or more cannabinoids in a cannabis plant, the method comprising down-regulating activity of tetrahydrocannabinolic acid (THCA) synthase in the cannabis plant, wherein the THCA synthase is expressed from a THCA synthase gene comprising a polynucleotide sequence selected from SEQ ID NOs: 1-28, 38, 40, 44-45, and 47;
(5)
(1)
|
|
7. A method for altering a content of one or more cannabinoids in a cannabis plant, the method comprising down-regulating activity of tetrahydrocannabinolic acid (THCA) synthase in the cannabis plant, wherein the THCA synthase is expressed from a THCA synthase gene comprising a polynucleotide sequence selected from SEQ ID NOs: 1-28, 38, 40, 44-45, and 47; wherein said down-regulating comprises:
(2)
(2)
|
|
10. A method for altering a content of one or more cannabinoids in a cannabis plant, the method comprising down-regulating activity of tetrahydrocannabinolic acid (THCA) synthase in the cannabis plant, wherein the THCA synthase is expressed from a THCA synthase gene comprising a polynucleotide sequence selected from SEQ ID NOs: 1-28, 38, 40, 44-45, and 47; wherein said down-regulating comprises:
(0)
(5)
|
|