Claim CLM-00001. 1. A kit for the detection Coronavirus polynucleotides in a biological sample comprising
(a) a primer pair, wherein at least one primer of the primer pair comprises the sequence of SEQ ID NO: 1, wherein at least one of the primers of the primer pair is modified with an internal spacer or detectable label; and wherein (a) is capable of detecting Coronavirus polynucleotides, if present, in the sample by recombinase polymerase amplification (RPA).
Claim CLM-00011. 11. A method for detecting Coronavirus polynucleotides in a biological sample comprising:
(a) an amplifying step comprising adding the biological sample to a vessel containing a primer pair that is capable of amplifying Corona virus polynucleotides, if present, in the biological sample, wherein at least one primer of the primer pair comprises the sequence of SEQ ID NO: 1, (b) combining the single-stranded amplified product with a running buffer comprising a capture probe that is capable of detecting the single-stranded amplified product to form a mixture, and incubating the mixture for a period of time in the vessel; and, (c) a detecting step comprising wicking the mixture into a test strip and visually detecting the capture probe on the test strip.
Claim CLM-00012. 12. A method for detecting Coronavirus polynucleotides in a biological sample comprising:
(a) an amplifying step comprising adding the biological sample to a vessel containing a primer pair that is capable of amplifying Coronavirus polynucleotides, if present, in the biological sample, wherein at least one primer of the primer pair comprises the sequence of SEQ ID NO: 1, (b) digesting amplified Coronavirus polynucleotides in the vessel into a single-stranded amplified product before the combing step; (c) combining the single-stranded amplified product with a running buffer comprising a capture probe that is capable of detecting the single-stranded amplified product to form a mixture, and incubating the mixture for a period of time in the vessel; and, (d) a detecting step comprising wicking the mixture into a test strip and visually detecting the capture probe on the test strip.
