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Application US20200107510
Published 2020-04-09
Generation Of New Varieties Of Cannabis By Chemical Mutagenesis Of Cannabis Cell Suspensions
An method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells includes subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants, suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells, centrifuging the mutated Cannabis cells to obtain pelleted cells, and providing the pelleted cells on culture media.
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- 1. A method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells, comprising:
subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants; suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells; centrifuging the mutated Cannabis cells to obtain pelleted cells; and providing the pelleted cells on culture media.
- 19. A method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells, comprising:
subjecting plant parts of Cannabis plants to pectinase treatment; incubating the pectinase-treated plant parts at a first temperature in a first incubation; filtering the incubated plant parts to obtain filtrate-containing cells; centrifuging the filtrate-containing cells in a first centrifuging operation; suspending the centrifuged filtrate-containing cells in a mutagenic solution that includes methane sulfonate (EMS) and dimethyl sulfoxide (DMSO); incubating the mutagenic solution at a second temperature after said suspending in a second incubation to obtain mutated Cannabis cells; centrifuging the mutagenic solution containing the mutated Cannabis cells in a second centrifuging operation to obtain pelleted cells; providing the pelleted cells in a series of culture media.
