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Application US20190316144
Published 2019-10-17
In Vivo Generation Of Water-soluble Cannabinoids In Plant Cell Suspension Cultures
The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble cannabinoids in plant cell suspension cultures.
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- 1. An in vivo method of producing water-soluble cannabinoids comprising the steps of:
genetically modifying a tobacco cell to express:
a nucleotide sequence encoding a heterologous cytochrome P450 operably linked to a promoter having hydroxylation or oxidation activity toward one or more cannabinoids to generate a hydroxylated cannabinoid or a cannabinoid carboxylic acid;
a nucleotide sequence encoding a heterologous P450 oxidoreductase operably linked to a promoter that facilitates electron transfer from nicotinamide adenine dinucleotide phosphate (NADPH) to the cytochrome P450;
wherein said tobacco cell expresses at least one endogenous glycosyltransferase protein having glycosylation activity towards one or more cannabinoids.
establishing a suspension cell culture of said genetically modified tobacco cells; introducing one or more cannabinoids to said suspension cell culture of said genetically modified tobacco cells; chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids; and isolating said water soluble glycosylated cannabinoids.
- 9. An in vivo method for the generation of water-soluble cannabinoids comprising the steps of:
establishing a suspension cell culture of tobacco cells that expresses at least one endogenous glycosyltransferase protein having glycosylation activity towards one or more cannabinoids; introducing one or more cannabinoids to said suspension cell culture of tobacco cells; and chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids through the action of said at least one endogenous glycosyltransferase.
- 20. A non-genetically modified in vivo method of producing water-soluble cannabinoids comprising the steps of:
establishing a plant cell suspension cell culture from a non-cannabinoid producing plant that expresses at least one endogenous glycosyltransferase having glycosylation activity towards one or more cannabinoids; introducing one or more cannabinoids to said plant cell suspension culture; chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids through the action of said at least one endogenous glycosyltransferase; isolating said glycosylated cannabinoids from said plant cell suspension culture.
- 23. A method of producing water-soluble cannabinoids comprising the steps of:
genetically modifying a plant cell to express a nucleotide sequence operably linked to a promoter encoding a heterologous glycosyltransferase from a tobacco plant having glycosylation activity towards one or more cannabinoids; establishing a suspension cell culture of said genetically modified plant cells; introducing one or more cannabinoids to said suspension cell culture of said genetically modified plant cells; chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids; and isolating said glycosylated cannabinoids from said plant cell suspension culture.
