Enjoy your
FREE PREVIEW which shows only 2022 data and 25 documents. Contact
Patent Forecast for full access.
AI Biotech/Diagnostics: General Diagnostics
Search All Patents in AI Biotech/Diagnostics: General Diagnostics
Patent US9976181
Issued 2018-05-22
Synthetic Nucleic Acid Spike-ins
This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.
Much More than Average Length Specification
View the Patent Matrix® Diagram to Explore the Claim Relationships
USPTO Full Text Publication >
- 1. A method for determining abundance of nucleic acids in an initial sample comprising target nucleic acids, the method comprising:
(a) adding a starting quantity of at least 1,000 unique synthetic nucleic acids to the initial sample, wherein each of the at least 1,000 unique synthetic nucleic acids comprises (i) an identifying tag and (ii) a variable region comprising at least 5 degenerate bases; (b) performing a sequencing assay on a portion of the target nucleic acids and on a portion of the at least 1,000 unique synthetic nucleic acids, thereby obtaining target and synthetic nucleic acid sequence reads; and (c) calculating a diversity loss value for the portion of the at least 1,000 unique synthetic nucleic acids, wherein abundance of the target nucleic acids in the initial sample is determined using the diversity loss value.
