Patent 9642317
Biotech Institute, Llc

Breeding, production, processing and use of specialty cannabis
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9642317

1. A cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescencecomprising: a) at least one B.sub.T allele, and at least one B.sub.D allele (13) (8)

b) cannabidiol (CBD) cannabinoid content of at least 1.5% by weight

c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than1.4

and d) a terpene oil content greater than about 1.0% by weight

wherein the contents of THC and CBD comprise acidic and decarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight ofthe inflorescence

wherein the terpene oil content is the additive content of the terpenes in the inflorescence's terpene profile

wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene,alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene

and wherein the terpene contents are measured by gas chromatography-flame ionizationdetection (GC-FID) and are calculated based on dry weight of the inflorescence

wherein a representative sample of seed capable of producing said plants has been deposited under NCIMB Nos. 42248, 42249, and wherein cellular cultures representative ofsaid plants are deposited under Bigelow NCMA Nos. PATENT201611001, PATENT201609001, and PATENT201612001.

2. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 wherein the inflorescence comprises a terpene profile in which myrcene is not the dominant terpene. (1) (0)

3. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the THC:CBD content ratio is greater than 1.5. (0)

4. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the THC:CBD content ratio is greater than 1.6. (0)

5. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the THC:CBD content ratio is greater than 1.7. (0)

6. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the THC:CBD content ratio is greater than 1.8. (0)

7. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the inflorescence comprises a CBD content that is at least 3.0% by weight. (0)

8. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the inflorescence comprises a THC content that is at least 6.0% by weight. (0)

9. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein the inflorescence comprises a CBD content that is at least 6.0% by weight. (0)

11. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B.sub.T allele, and at least one B.sub.D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratiohigher than 1.4, and a terpene oil content greater than about 1.0% by weight, said method comprising: (i) making a cross between a first cannabis plant of claim 1, or an asexual clone of said first cannabis plant and a second cannabis plant to produce anF1 seed: (ii) harvesting the resulting seed (0) (3)

12. A method of producing a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B.sub.T allele, and at least one B.sub.D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higherthan 1.4, and a terpene oil content greater than about 1.0% by weight, said method comprising: (i) obtaining a cannabis seed, cutting, or plant cell, from a cannabis plant, or an asexual clone of said cannabis plant of claim 1 (0) (3)

13. A cannabis extract from the cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1. (2) (0)

15. An edible product comprising cannabis tissue from the cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1. (0)

17. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein said plant part is selected from the group consisting of: trichomes, sun leaves, or inflorescences. (0)

18. A progeny plant derived from the cross of SIL08.times.CBD05, said progeny plant being capable of producing a female inflorescence, said inflorescence comprising: a) at least one B.sub.T allele, and at least one B.sub.D allele (0) (4)

b) acannabidiol (CBD) cannabinoid content of at least 1.5% by weight

and c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than 1.4

and wherein the contents of THC and CBD comprise acidic anddecarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence

wherein seed of said progeny plants has been deposited under NCIMB No. 42248.

19. A cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescence comprising: a) at least one B.sub.T allele, and at least one B.sub.D allele (1) (9)

b) cannabidiol (CBD) cannabinoid content of at least 1.5% by weight

c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than 1.4

d) a terpene oil content greater than about 1.0% by weight

and e) aterpene profile in which myrcene is not the dominant terpene

wherein the contents of THC and CBD comprise acidic and decarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of theinflorescence

wherein the terpene oil content is the additive content of the terpenes in the inflorescence's terpene profile

and wherein the terpene contents are measured by gas chromatography-flame ionizationdetection (GC-FID) and are calculated based on dry weight of the inflorescence

wherein a representative sample of seed capable of producing said plants has been deposited under NCIMB No. 42249, and wherein cellular cultures representative of saidplants are deposited under Bigelow NCMA Nos. PATENT201611001, and PATENT201609001.

20. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B.sub.T allele and at least one B.sub.D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratiohigher than 1.4, a terpene oil content greater than about 1.0% by weight, and a terpene profile in which myrcene is not the dominant terpene, said method comprising: (i) making a cross between a first cannabis plant of claim 19, or an asexual clone ofsaid first cannabis plant and a second cannabis plant to produce an F1 seed: (ii) harvesting the resulting seed (0) (3)

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Patent 9642317 Biotech Institute, Llc

Breeding, production, processing and use of specialty cannabis

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Patent 9642317
Biotech Institute, Llc