Patent 10582676
Biotech Institute, Llc

Breeding, production, processing and use of specialty cannabis
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10582676

1. A cannabis plant YEL03xTP05, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, saidinflorescence comprising: a) a terpene profile in which myrcene is not the dominant terpene (5) (8)

b) a terpene oil content greater than 1.0%

c) a propyl cannabinoid content of at least 1.0%

d) a B.sub.T/B.sub.T genotype

and e) a terpene profile inwhich beta caryophyllene is the most abundant terpene

wherein the propyl cannabinoid content is defined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), andcannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence

wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gammaterpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant, and wherein the terpene oil content is determined by the additivecontent of the terpenes in the terpene profile

wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence

and wherein cellular cultures of said plant aredeposited under Bigelow NCMA Accession No. 201912001.

2. The cannabis plant, or an asexual clone of said cannabis plant, or plant part, tissue, or cell thereof of claim 1, wherein the female inflorescence comprises a propyl cannabinoid content of at least 3.0%. (0)

3. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, and a terpene profile in which myrcene is notthe dominant terpene, said method comprising: (i) making a cross between a first cannabis plant of claim 1, or an asexual clone of said first cannabis plant and a second cannabis plant to produce an F1 seed: (ii) harvesting the resulting seed (0) (3)

4. A method of producing a cannabis plant, wherein said cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, a terpene profile in which myrcene is not thedominant terpene, a B.sub.T/B.sub.T genotype, and a terpene profile in which beta caryophyllene is the most abundant terpene, said method comprising: (i) obtaining a cannabis seed, cutting, or plant cell, from a cannabis plant, or an asexual clone ofsaid cannabis plant of claim 1 (0) (3)

5. An edible product comprising cannabis tissue from the inflorescence of claim 1. (0)

6. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1, wherein said plant part is selected from the group consisting of: trichomes, sun leaves, or inflorescences. (0)

7. A regenerable cell from a cannabis plant designated YEL03xTP05, wherein cellular cultures of said plant are deposited under Bigelow NCMA Accession No. 201912001. (1) (0)

8. A cannabis plant, or plant part, tissue, or cell thereof produced by regenerating the cell of claim 7, wherein said cannabis plant, or plant part, tissue, or cell thereof comprises a cannabinoid profile and a terpene profile as set forth inTables 43 and 44, respectively. (4) (0)

9. The cannabis plant part of claim 8, wherein said plant part is selected from the group consisting of: stems, trichomes, leaves, and flower buds. (0)

10. A cannabis clone regenerated from the cannabis plant of claim 8. (0)

11. An edible product comprising the cannabis plant, or plant part, tissue, or cell thereof of claim 8. (0)

12. A method of producing an F1 cannabis seed, said method comprising crossing the plant of claim 8 with a different cannabis plant, and harvesting the resultant F1 cannabis seed. (1) (0)

13. The F1 hybrid cannabis seed produced by the method of claim 12. (1) (0)

14. An F1 hybrid cannabis plant produced by growing the F1 hybrid cannabis seed of claim 13, wherein said F1 hybrid plant produces a female inflorescence, said inflorescence comprising: a) a terpene profile in which myrcene is not the dominantterpene (2) (8)

b) a terpene oil content greater than 1.0%

c) a propyl cannabinoid content of at least 1.0%

d) a B.sub.T/B.sub.T genotype

and e) a terpene profile in which beta caryophyllene is the most abundant terpene

wherein the propyl cannabinoidcontent is defined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), and cannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) andcalculated based on dry weight of the inflorescence

wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alphaterpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant

wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile

and wherein the terpene contents aremeasured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence.

15. A cannabis clone regenerated from the F1 hybrid cannabis plant of claim 14, wherein said cannabis clone produces a female inflorescence, said inflorescence comprising: a) a terpene profile in which myrcene is not the dominant terpene (0) (8)

b) aterpene oil content greater than 1.0%

c) a propyl cannabinoid content of at least 1.0%

d) a B.sub.T/B.sub.T genotype

and e) a terpene profile in which beta caryophyllene is the most abundant terpene

wherein the propyl cannabinoid content isdefined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), and cannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) and calculatedbased on dry weight of the inflorescence

wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alphahumulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant

wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile

and wherein the terpene contents are measured by gaschromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence.

16. An edible product comprising cannabis tissue from the F1 hybrid cannabis plant of claim 14. (0)

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Patent 10582676 Biotech Institute, Llc

Breeding, production, processing and use of specialty cannabis

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Patent 10582676
Biotech Institute, Llc