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Application 20210040512
Baymedica, Inc.

Recombinant Production Systems For Prenylated Polyketides Of The Cannabinoid Family

The present invention relates generally to production methods, enzymes and recombinant yeast strains for the biosynthesis of clinically important prenylated polyketides of the cannabinoid family. Using readily available starting materials, heterologous enzymes are used to direct cannabinoid biosynthesis in yeast.

Much More than Average Length Specification


1 Independent Claims

  • Claim 1. A modified recombinant host cell comprising: (i) a first exogenous polynucleotide that encodes an acyl-CoA synthetase that converts an aliphatic carboxylic acid to an acyl CoA thioester, wherein the acyl-CoA synthetase is a revS polypeptide or a CsAAE3 polypeptide(ii) a second exogenous polynucleotide that encodes olivetolic acid synthase, (iii) a third exogenous polynucleotide that encodes a 2-alkyl-4,6-dihydroxybenzoic acid cyclase(iv) a fourth exogenous polynucleotide that encodes a prenyltransferase that catalyzes coupling of geranyl-pyrophosphate to a 2-alkyl-4,6-dihydroxybenzoic acid to produce an acidic cannabinoidand one or more additional exogenous polynucleotides selected from: an exogenous polynucleotide that encodes a prenol and isoprenol kinasean exogenous polynucleotide that encodes a kinase that produces dimethylallyl pyrophosphate and isopentenyl pyrophosphate when grown in the presence of exogenous prenol and isoprenoland an exogenous polynucleotide that encodes a geranyl-pyrophosphate synthase.
  • Claim 10. A modified recombinant host cell comprising: (i) a first exogenous polynucleotide that encodes prenol and isoprenol kinase(ii) a second exogenous polynucleotide that encodes kinase activity to produce dimethylallyl pyrophosphate and isopentenyl pyrophosphate when grown in the presence of exogenous prenol and isoprenol(iii) a third exogenous polynucleotide that encodes a geranyl-pyrophosphate synthaseand (iv) a fourth exogenous polynucleotide that encodes a prenyltransferase that catalyzes coupling of geranyl-pyrophosphate to a 2-alkyl-4,6-dihydroxybenzoic acid to form an acidic cannabinoid.