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Patent US10982243

Methods for increasing production of cannabinoids in yeast cells

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1. A method for increasing production of a cannabinoid or cannabinoid precursor molecule, comprising: providing a genetically modified yeast cell comprising: (i) a codonoptimized polynucleotide sequence expressing cannabinoid or cannabinoid precursor molecule producing enzymes, and (ii) a mutation to prevent or reduce endogenous sterol synthesis selected from the group consisting of: a SUE (sterol uptake exogenous)mutation and an ERG1 (Squalene monooxygenase) gene knockout (7) (2)

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