Patent US10378020

genetically modifying a cultured Cannabis cell to transiently express: i. a nucleotide sequence encoding at least one cannabinoid synthase having its trichome targeting signal disrupted or removed; ii. a nucleotide sequence encoding a heterologous cytochrome P450 having hydroxylation or oxidation activity toward a cannabinoid to generate a hydroxylated cannabinoid or a cannabinoid carboxylic acid; iii. a nucleotide sequence encoding a heterologous P450 oxidoreductase that facilitates electron transfer from nicotinamide adenine dinucleotide phosphate (NADPH) to the cytochrome P450; and iv. a nucleotide sequence encoding a heterologous glycosyltransferase having glycosylation activity towards said cannabinoids, said hydroxylated cannabinoids, and said cannabinoid carboxylic acids.

genetically modifying at least one Cannabis plant through Agrobacterium-mediated transformation to transiently express: i. a nucleotide sequence encoding at least one cannabinoid synthase having its trichome targeting signal disrupted or removed; ii. a nucleotide sequence encoding a heterologous cytochrome P450 having hydroxylation or oxidation activity towards a cannabinoid to generate a hydroxylated cannabinoid, or a cannabinoid carboxylic acid; iii. a nucleotide sequence encoding a heterologous P450 oxidoreductase that facilitates electron transfer from NADPH to the cytochrome P450; and iv. a nucleotide sequence encoding a heterologous glycosyltransferase having glycosylation activity towards said cannabinoids, said hydroxylated cannabinoids, and said cannabinoid carboxylic acids.

 

genetically modifying at least one Cannabis plant cell through Agrobacterium-mediated transformation to transiently express: i. a nucleotide sequence encoding at least one cannabinoid synthase having its trichome targeting signal disrupted or removed; ii. a nucleotide sequence encoding a heterologous cytochrome P450 having hydroxylation or oxidation activity towards a cannabinoid to generate a hydroxylated cannabinoid, or a cannabinoid carboxylic acid; iii. a nucleotide sequence encoding a heterologous P450 oxidoreductase that facilitates electron transfer from NADPH to the cytochrome P450; and iv. a nucleotide sequence encoding a heterologous glycosyltransferase having glycosylation activity towards said cannabinoids, said hydroxylated cannabinoids, and said cannabinoid carboxylic acids; v. a nucleotide sequence encoding an endogenous or heterologous myb transcription factor wherein said heterologous or heterologous myb transcription factor increases cannabinoid production by enhancing metabolite flux through the cannabinoid biosynthetic pathway; and vi. a nucleotide sequence encoding a heterologous catalase having reduction activity towards hydrogen peroxide generated by cannabinoid biosynthesis.