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Application US20200165641
Published 2020-05-28
Bidirectional Multi-enzymatic Scaffolds For Biosynthesizing Cannabinoids
This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.
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- 1. A host cell capable of producing one or more cannabinoids selected from the group consisting of cannabigerolic acid, cannabidiolic acid, and cannabichromenic acid, said host cell comprising at least three different exogenous nucleic acids,
wherein said first and said second exogenous nucleic acids each encode a plurality of engineered enzymes selected from the group consisting of an acetyl-CoA acetyltransferase, a 3-hydroxybutyryl-CoA dehydrogenase, an enoyl-CoA hydratase, a beto-ketothiolase, a trans-enoyl-CoA reductase, an HMG-CoA synthase, an HMG-CoA reductase, a mevalonate kinase, a phosphomevalonate kinase, a diphosphomevalonate decarboxylase, an isopentenyl-diphosphate delta isomerase, a geranyl-diphosphate synthase, an olivetol synthase, an olivetolic acid cyclase, and a CBGA synthase; wherein each of said engineered enzymes comprises a heterologous interaction domain, said heterologous interaction domain comprising a first and a second peptide motif, and wherein each said heterologous interaction domain is different from each other; and wherein said third exogenous nucleic acid encodes a polypeptide scaffold comprising a plurality of peptide ligands, wherein each said peptide ligand comprises an amino acid sequence that can bind to said first or said second peptide motif of one of said heterologous interaction domains.
